E is the third note of the C major scale, and mi in fixed-do solfège. It has enharmonic equivalents of F♭ (which is by definition a diatonic semitone above E♭) and D, amongst others. When calculated in equal temperament with a reference of A above middle C as 440 Hz, the frequency of Middle E (E4) is approximately 329.628 Hz. See pitch (music) for a discussion of historical variations in frequency.
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