An agar plate is a sterile Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms. Selective growth compounds may also be added to the media, such as antibiotics.

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  • An agar plate is a sterile Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms. Selective growth compounds may also be added to the media, such as antibiotics. Individual microorganisms placed on the plate will grow into individual colonies, each a clone genetically identical to the individual ancestor organism (except for the low, unavoidable rate of mutation). Thus, the plate can be used either to estimate the concentration of organisms in a liquid culture or a suitable dilution of that culture, using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms, using a technique known as streaking. In this technique, a drop of the culture on the end of a thin, sterile loop of wire is "streaked" across the surface of the agar leaving organisms behind, a higher number at the beginning of the streak and a lower number at the end. At some point during a successful "streak", the number of organisms deposited will be such that distinct individual colonies will grow in that area which may be removed for further culturing, using another sterile loop. Agar plates may be formulated as either permissive, with the intent of allowing the growth of whatever organisms are present, or restrictive or selective, with the intent of only selecting for growth a particular subset of those organisms. This may take the form of a nutritional requirement, for instance providing a particular compound such as lactose as the only source of carbon for energy and material and thereby selecting only organisms which can metabolize that compound, or by including a particular antibiotic or other substance in order to select only organisms which are resistant to that substance. This correlates to some degree with defined and undefined media; undefined media, made from natural products and containing an unknown combination of very many organic molecules, is typically more permissive in terms of supplying the needs of a wider variety of organisms, while defined media can be precisely tailored to select organisms with very specific properties. Agar plates may also be indicator plates, where the organisms are not selected on the basis of growth, but a compound in the agar is altered by an enzyme or similar in some colonies so as to change color and identify them from those lacking the enzyme. Some commonly used agar plate types are: (en)
  • 寒天培地（かんてんばいち、Agar medium）とは、寒天を用いた培地のこと。特に、寒天を約1.5%の濃度で加えて固化させた固形培地のことを意味する場合が多い。微生物学や植物学の分野で、微生物や細胞を培養するために用いられる。用途において様々な種類がある。 (ja)
  • Płytka agarowa - sterylna szalka Petriego zawierająca podłoże hodowlane (przeważnie agarowe), używana do hodowli kultur mikroorganizmów. Możliwe jest również dodanie selektywnych czynników wzrostu, takich jak antybiotyki. Zawarte na szalce indywidualne drobnoustroje uformują się w indywidualne kolonie, z których każda będzie identycznym genetycznie klonem przodka (z wyjątkiem niskiego, nieuniknionego wskaźnika mutacji). Płytki można użyć do oszacowania koncentracji organizmów w kulturze płynnej lub w odpowiednim rozcieńczeniu takiej kultury, używając licznika kolonii. Możliwa jest też generacja kultur czystych z mieszanej kultury organizmów genetycznie różnych, w metodzie zwanej hodowlą pasmową. (pl)
  • 瓊脂平板（Agar Plate）是一種把消毒後的培養基加上繁殖微生物所需村料（通常是洋菜及養分）後製成的有蓋培養皿。由於提供了微生物繁殖的有利條件，瓊脂平板能夠用來種出不同的微生物。某些特定協助生長用的物質有時也會用到，例如抗生素等。另外在瓊脂平板上的細菌因為不受干擾，因此繁演的菌落從生物學的角度看每個細菌都是一樣的，因此這些細菌在醫學或生物學的研究才不會有誤差。 (zh)
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  • An agar plate streaked with microorganisms isolated from a deep-water sponge. Individual colonies may be seen center right. (en)
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  • Agar plate (en)
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  • An agar plate is a sterile Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms. Selective growth compounds may also be added to the media, such as antibiotics. (en)
  • 寒天培地（かんてんばいち、Agar medium）とは、寒天を用いた培地のこと。特に、寒天を約1.5%の濃度で加えて固化させた固形培地のことを意味する場合が多い。微生物学や植物学の分野で、微生物や細胞を培養するために用いられる。用途において様々な種類がある。 (ja)
  • Płytka agarowa - sterylna szalka Petriego zawierająca podłoże hodowlane (przeważnie agarowe), używana do hodowli kultur mikroorganizmów. Możliwe jest również dodanie selektywnych czynników wzrostu, takich jak antybiotyki. (pl)
  • 瓊脂平板（Agar Plate）是一種把消毒後的培養基加上繁殖微生物所需村料（通常是洋菜及養分）後製成的有蓋培養皿。由於提供了微生物繁殖的有利條件，瓊脂平板能夠用來種出不同的微生物。某些特定協助生長用的物質有時也會用到，例如抗生素等。另外在瓊脂平板上的細菌因為不受干擾，因此繁演的菌落從生物學的角度看每個細菌都是一樣的，因此這些細菌在醫學或生物學的研究才不會有誤差。 (zh)
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  • Agar plate (en)
  • 寒天培地 (ja)
  • Płytka agarowa (pl)
  • 瓊脂平板 (zh)
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