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The women's sprint cross-country skiing competition at the FIS Nordic World Ski Championships 2009 was held on 24 February 2009. The Sprint qualifying began at 11:35 CET with finals at 13:30 CET. The defending world champion was Norway's Astrid Uhrenholdt Jacobsen, but she was eliminated in the qualification round at these championships. It was the first individual gold medal for Arianna Follis.