The Great Grimsby and Sheffield Junction Railway was an early British railway company which existed between 1845 and 1847 with the intention of providing rail services between Grimsby, New Holland and Gainsborough in the county of Lincolnshire. It amalgamated with the Sheffield, Ashton-Under-Lyne and Manchester Railway and the Sheffield and Lincolnshire Junction Railway, the three being renamed the Manchester, Sheffield and Lincolnshire Railway in 1847.