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- Propidiumiodid (PI) wirkt wie Ethidiumbromid als Nukleinsäureinterkalator. Der Farbstoff kann die perforierte Zellmembran von toten Zellen, jedoch nicht die intakte Membran von lebenden Zellen durchdringen. Diese Eigenschaft wird in der Durchflusszytometrie zur Diskriminierung der Zellviabilität verwendet. Eine weitere durchflusszytometrische Anwendung von PI stellt die Messung des DNA-Gehaltes von Zellen dar. Dies wird vor allem zur Analyse des Zellzyklus oder zur Bestimmung von Polyploidie eingesetzt. Propidiumiodid hat ein Absorptionsmaximum bei 488 nm und ein Emissionsmaximum bei 590 nm. Diese Maxima verschieben sich zu 535 nm beziehungsweise 617 nm, wenn Propidiumiodid in DNA interkaliert. (de)
- Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane integrity. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining. PI is widely used in fluorescence staining and visualization of the plant cell wall. (en)
- L'iodure de propidium (IP, en anglais propidium iodide, PI) est un agent intercalant des acides nucléiques (l'ADN ou l'ARN) et une molécule fluorescente ayant une masse moléculaire de 668,403 Da. L'iodure de propidium est couramment utilisé comme marqueur de l'ADN afin de marquer le noyau des cellules ayant perdu leur intégrité membranaire (phénomène caractéristique de la nécrose). (fr)
- 프로피디움 아이오다이드(Propidium iodide, PI)는 세포를 염색하는데 사용할 수 있는 형광 물질이다. PI는 세포분석기(flow cytometry)로 세포의 생존 능력(cell viability)을 평가 할 때나, 세포 주기 분석(cell cycle analysis)을 할 때 DNA 내용물을 평가할때, DNA를 염색(PI stain)하기 위해 사용한다. 또한 현미경 상에서 핵과 다른 DNA포함 세포소기관을 관찰하기 위해 사용한다. PI는 살아있는 세포의 세포막을 통과하지 못하기 때문에, 괴저성 또는 세포자살 세포로부터 건강한 세포를 구별하는데 이용할 수 있다. PI는 또한 RNA에도 결합하므로, RNA와 DNA 염색을 구분하기 위해서는 핵산분해효소(nuclease) 처리가 필요하다. (ko)
- 碘化丙啶（英語：Propidium iodide，缩写PI），是一种能结合DNA的荧光染料，广泛应用于流式細胞術，在游离状态下激发峰在495nm，发射峰在519nm，在结合DNA后，荧光的增强20到30倍，激发峰和发射峰分别迁移到535nm和617nm。 (zh)
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- L'iodure de propidium (IP, en anglais propidium iodide, PI) est un agent intercalant des acides nucléiques (l'ADN ou l'ARN) et une molécule fluorescente ayant une masse moléculaire de 668,403 Da. L'iodure de propidium est couramment utilisé comme marqueur de l'ADN afin de marquer le noyau des cellules ayant perdu leur intégrité membranaire (phénomène caractéristique de la nécrose). (fr)
- 프로피디움 아이오다이드(Propidium iodide, PI)는 세포를 염색하는데 사용할 수 있는 형광 물질이다. PI는 세포분석기(flow cytometry)로 세포의 생존 능력(cell viability)을 평가 할 때나, 세포 주기 분석(cell cycle analysis)을 할 때 DNA 내용물을 평가할때, DNA를 염색(PI stain)하기 위해 사용한다. 또한 현미경 상에서 핵과 다른 DNA포함 세포소기관을 관찰하기 위해 사용한다. PI는 살아있는 세포의 세포막을 통과하지 못하기 때문에, 괴저성 또는 세포자살 세포로부터 건강한 세포를 구별하는데 이용할 수 있다. PI는 또한 RNA에도 결합하므로, RNA와 DNA 염색을 구분하기 위해서는 핵산분해효소(nuclease) 처리가 필요하다. (ko)
- 碘化丙啶（英語：Propidium iodide，缩写PI），是一种能结合DNA的荧光染料，广泛应用于流式細胞術，在游离状态下激发峰在495nm，发射峰在519nm，在结合DNA后，荧光的增强20到30倍，激发峰和发射峰分别迁移到535nm和617nm。 (zh)
- Propidiumiodid (PI) wirkt wie Ethidiumbromid als Nukleinsäureinterkalator. Der Farbstoff kann die perforierte Zellmembran von toten Zellen, jedoch nicht die intakte Membran von lebenden Zellen durchdringen. Diese Eigenschaft wird in der Durchflusszytometrie zur Diskriminierung der Zellviabilität verwendet. Eine weitere durchflusszytometrische Anwendung von PI stellt die Messung des DNA-Gehaltes von Zellen dar. Dies wird vor allem zur Analyse des Zellzyklus oder zur Bestimmung von Polyploidie eingesetzt. (de)
- Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic (en)
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- Propidiumiodid (de)
- Iodure de propidium (fr)
- 프로피듐 아이오다이드 (ko)
- Propidium iodide (en)
- 碘化丙啶 (zh)
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