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- Die Bimolekulare Fluoreszenzkomplementation (engl. bimolecular fluorescence complementation, BiFC) ist ein Verfahren der Molekularbiologie zum Nachweis von Protein-Protein-Interaktionen. Das Verfahren basiert auf der Komplementation zweier nicht fluoreszierender Fragmente eines fluoreszierenden Proteins, wie beispielsweise grün fluoreszierendes Protein (GFP). Durch Zusammenlagerung der beiden Fragmente wird ein intakter, fluoreszierender Komplex erzeugt. Das Verfahren der bimolekularen Fluoreszenzkomplementation wurde maßgeblich von und Mitarbeitern entwickelt. (de)
- Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest. (en)
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- Die Bimolekulare Fluoreszenzkomplementation (engl. bimolecular fluorescence complementation, BiFC) ist ein Verfahren der Molekularbiologie zum Nachweis von Protein-Protein-Interaktionen. Das Verfahren basiert auf der Komplementation zweier nicht fluoreszierender Fragmente eines fluoreszierenden Proteins, wie beispielsweise grün fluoreszierendes Protein (GFP). Durch Zusammenlagerung der beiden Fragmente wird ein intakter, fluoreszierender Komplex erzeugt. Das Verfahren der bimolekularen Fluoreszenzkomplementation wurde maßgeblich von und Mitarbeitern entwickelt. (de)
- Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the f (en)
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- Bimolekulare Fluoreszenzkomplementation (de)
- Bimolecular fluorescence complementation (en)
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