Cas12a (CRISPR associated protein 12a, previously known as Cpf1) is an RNA-guided endonuclease of that forms part of the CRISPR system in some bacteria and is used by scientists to modify DNA. It originates as part of a bacterial immune mechanism, where it serves to destroy the genetic material of viruses and thus protect the cell and colony from viral infection. Cas12a and other CRISPR associated endonucleases are unique in that their use of a guide RNA makes this action highly selective; they only cut DNA when it is adjacent to a certain sequence of nucleotides. In the organisms from which it originates, this guide RNA is a copy of a piece of the genome from a virus that previously infected the cell.
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| - CRISPR/Cpf1-Methode (de)
- CRISPR/Cpf1 (es)
- Cas12a (en)
- CRISPR/Cpf1 (pt)
- CRISPR/Cpf1 (zh)
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| - Das CRISPR/Cpf1-Methode (englisch Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella) ist eine biochemische Methode zur Erzeugung von gentechnisch veränderten Organismen. (de)
- Repetições Palindrômicas Curtas Agrupadas e Regularmente Interespaçadas de Prevotella e Francisella 1 ou CRISPR/Cpf1 é uma tecnologia de edição de DNA análoga ao sistema CRISPR/Cas9. Cpf1 é uma endonuclease guiada por RNA de um sistema CRISPR/Cas de classe II. Este mecanismo imunológico adquirido é encontrado nas bactérias Prevotella e Francisella e permite edição genômica eficiente, incluindo edição livre de DNA em plantas, com maior eficiência, especificidade e aplicações potencialmente mais amplas do que CRISPR/Cas9. CRISPR/Cpf1 explora vias de reparo dirigidas por homologia dentro de células intactas de mamíferos. (pt)
- CRISPR/Cpf1 是一种DNA编辑的技术，其原理与 CRISPR/Cas 系统类似。CRISPR/Cpf1 也藉由細菌或古菌对噬菌体的获得性免疫机制来进行基因编辑。由于 Cpf1 蛋白是一种比 Cas9 蛋白更小而且更简单的核酸内切酶，这样把CRISPR/Cpf1系统植入待修改基因的细胞就相对更容易，CRISPR/Cpf1可能是一种比CRISPR/Cas9更好的基因编辑工具。 (zh)
- Cas12a (CRISPR associated protein 12a, previously known as Cpf1) is an RNA-guided endonuclease of that forms part of the CRISPR system in some bacteria and is used by scientists to modify DNA. It originates as part of a bacterial immune mechanism, where it serves to destroy the genetic material of viruses and thus protect the cell and colony from viral infection. Cas12a and other CRISPR associated endonucleases are unique in that their use of a guide RNA makes this action highly selective; they only cut DNA when it is adjacent to a certain sequence of nucleotides. In the organisms from which it originates, this guide RNA is a copy of a piece of the genome from a virus that previously infected the cell. (en)
- CRISPR/Cpf1(en inglés: Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1, en español repeticiones palindrómicas cortas agrupadas y regularmente interespaciadas de Prevotella y Francisella 1) es una tecnología que edita el ADN análogo al sistema CRISPR/Cas9, caracterizado en 2015 por el grupo de Feng Zhang‘s del Instituto Broad y MIT. Cpf1 es un ARN-guiado de endonucleasa de clase II del sistema CRISPR/Cas. Este mecanismo inmune adquirido se encuentra en bacterias Prevotella y Francisella. Impide el daño genético provocados por virus. Los genes Cpf1 están asociados con el locus de CRISPR, codificando para una endonucleasa que usa un ARN guía para encontrar y cortar ADN viral. Cpf1 es una endonucleasa más pequeña y más sencillo que Cas9, venciendo a (es)
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| - Das CRISPR/Cpf1-Methode (englisch Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella) ist eine biochemische Methode zur Erzeugung von gentechnisch veränderten Organismen. (de)
- Cas12a (CRISPR associated protein 12a, previously known as Cpf1) is an RNA-guided endonuclease of that forms part of the CRISPR system in some bacteria and is used by scientists to modify DNA. It originates as part of a bacterial immune mechanism, where it serves to destroy the genetic material of viruses and thus protect the cell and colony from viral infection. Cas12a and other CRISPR associated endonucleases are unique in that their use of a guide RNA makes this action highly selective; they only cut DNA when it is adjacent to a certain sequence of nucleotides. In the organisms from which it originates, this guide RNA is a copy of a piece of the genome from a virus that previously infected the cell. It is of interest to researchers because it can be used to make highly targeted modifications of DNA or RNA, similar to the better known CRISPR/Cas9 system. Cas12a is distinguished from Cas9 by being smaller and simpler, not requiring a tracrRNA, and creating sticky rather than blunt ends at the cut site. These and other differences may make it more suitable in certain applications. Beyond its use in basic research, CRISPR/Cas12a could have applications in the treatment of genetic illnesses and in implementing gene drives. (en)
- CRISPR/Cpf1(en inglés: Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1, en español repeticiones palindrómicas cortas agrupadas y regularmente interespaciadas de Prevotella y Francisella 1) es una tecnología que edita el ADN análogo al sistema CRISPR/Cas9, caracterizado en 2015 por el grupo de Feng Zhang‘s del Instituto Broad y MIT. Cpf1 es un ARN-guiado de endonucleasa de clase II del sistema CRISPR/Cas. Este mecanismo inmune adquirido se encuentra en bacterias Prevotella y Francisella. Impide el daño genético provocados por virus. Los genes Cpf1 están asociados con el locus de CRISPR, codificando para una endonucleasa que usa un ARN guía para encontrar y cortar ADN viral. Cpf1 es una endonucleasa más pequeña y más sencillo que Cas9, venciendo algunas de las limitaciones del sistema CRISPR/Cas9. CRISPR/Cpf1 podría tener aplicaciones múltiples, incluyendo el tratamiento de enfermedades genéticas y condiciones degenerativas.Actualmente la nucleasa Cpf1 recibe el nombre de Cas12a, pasando a denominar a este sistema CRISPR/Cas12a. (es)
- Repetições Palindrômicas Curtas Agrupadas e Regularmente Interespaçadas de Prevotella e Francisella 1 ou CRISPR/Cpf1 é uma tecnologia de edição de DNA análoga ao sistema CRISPR/Cas9. Cpf1 é uma endonuclease guiada por RNA de um sistema CRISPR/Cas de classe II. Este mecanismo imunológico adquirido é encontrado nas bactérias Prevotella e Francisella e permite edição genômica eficiente, incluindo edição livre de DNA em plantas, com maior eficiência, especificidade e aplicações potencialmente mais amplas do que CRISPR/Cas9. CRISPR/Cpf1 explora vias de reparo dirigidas por homologia dentro de células intactas de mamíferos. (pt)
- CRISPR/Cpf1 是一种DNA编辑的技术，其原理与 CRISPR/Cas 系统类似。CRISPR/Cpf1 也藉由細菌或古菌对噬菌体的获得性免疫机制来进行基因编辑。由于 Cpf1 蛋白是一种比 Cas9 蛋白更小而且更简单的核酸内切酶，这样把CRISPR/Cpf1系统植入待修改基因的细胞就相对更容易，CRISPR/Cpf1可能是一种比CRISPR/Cas9更好的基因编辑工具。 (zh)
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