An Entity of Type: protein, from Named Graph: http://dbpedia.org, within Data Space: dbpedia.org:8891

T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme. It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase.

Property Value
dbo:abstract
  • T4-DNA-Polymerase ist ein Enzym aus der Gruppe der DNA-Polymerasen, das vom Gen 43 des Bakteriophagen T4 codiert wird. (de)
  • T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme. It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. This polymerase has various applications in site-directed mutagenesis as well as a high-fidelity enzyme suitable for PCR. It has also served as the precursor to Sequenase, an engineered-enzyme optimized for DNA sequencing. (en)
dbo:thumbnail
dbo:wikiPageExternalLink
dbo:wikiPageID
  • 19309200 (xsd:integer)
dbo:wikiPageLength
  • 22099 (xsd:nonNegativeInteger)
dbo:wikiPageRevisionID
  • 1067003427 (xsd:integer)
dbo:wikiPageWikiLink
dbp:caption
  • Figure 1. Crystal structure of T7 DNA replication complex. Rendered from PDB 1T7P. (en)
dbp:casNumber
  • 9012 (xsd:integer)
dbp:ecnumber
  • 2.700000 (xsd:double)
dbp:name
  • DNA-directed DNA polymerase (en)
  • Nucleotide kinase (en)
  • Single-stranded DNA-binding protein (en)
dbp:organism
  • Enterobacteria phage T7 (en)
dbp:symbol
  • 1.700000 (xsd:double)
  • 2.500000 (xsd:double)
  • 5 (xsd:integer)
dbp:uniprot
  • P00581 (en)
  • P03696 (en)
  • P03781 (en)
dbp:wikiPageUsesTemplate
dcterms:subject
gold:hypernym
rdf:type
rdfs:comment
  • T4-DNA-Polymerase ist ein Enzym aus der Gruppe der DNA-Polymerasen, das vom Gen 43 des Bakteriophagen T4 codiert wird. (de)
  • T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme. It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. (en)
rdfs:label
  • T4-DNA-Polymerase (de)
  • T7 DNA polymerase (en)
owl:sameAs
prov:wasDerivedFrom
foaf:depiction
foaf:isPrimaryTopicOf
is dbo:wikiPageWikiLink of
is foaf:primaryTopic of
Powered by OpenLink Virtuoso    This material is Open Knowledge     W3C Semantic Web Technology     This material is Open Knowledge    Valid XHTML + RDFa
This content was extracted from Wikipedia and is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License