About: NgAgo

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NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM).

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  • NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). (en)
  • NgAgo é uma endonuclease de guiada por DNA de cadeia simples (ssDNA), um acrônimo para Argonauta Natronobacterium gregoryi. O NgAgo liga o ssDNA fosforilado em 5 'de ~ 24 nucleotídeos (gDNA) para guiá-lo ao seu local alvo e fará quebras de fita dupla no DNA no local do gDNA. Como o sistema CRISPR/Cas, o NgAgo foi descrito como adequado para edição de genoma, mas isso não foi replicado. Ao contrário do Cas9, o sistema NgAgo – gDNA não requer um motivo adjacente protospacer (PAM). (pt)
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  • NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for Natronobacterium gregoryi Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). (en)
  • NgAgo é uma endonuclease de guiada por DNA de cadeia simples (ssDNA), um acrônimo para Argonauta Natronobacterium gregoryi. O NgAgo liga o ssDNA fosforilado em 5 'de ~ 24 nucleotídeos (gDNA) para guiá-lo ao seu local alvo e fará quebras de fita dupla no DNA no local do gDNA. Como o sistema CRISPR/Cas, o NgAgo foi descrito como adequado para edição de genoma, mas isso não foi replicado. Ao contrário do Cas9, o sistema NgAgo – gDNA não requer um motivo adjacente protospacer (PAM). (pt)
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  • NgAgo (en)
  • NgAgo (pt)
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