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- Das HA-Tag ist ein Protein-Tag, der bei der Erzeugung und Charakterisierung von rekombinanten Proteinen verwendet wird. (de)
- Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA-tag, which does not generally appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation and purification of the protein of interest. The HA-tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity. Labeling of endogenous proteins with HA-tag using CRISPR was recently accomplished in-vivo in differentiated neurons. (en)
- HA標籤(HA-tag)是一種基於人流感病毒血凝素(Human influenza hemagglutinin)抗原的蛋白標籤,其化學本質爲一段來自人流感病毒血凝素98-106號氨基酸的短氨基酸序列。使用分子生物學手段將HA標籤序列接合到目的蛋白的一端後,就可以使用抗HA標籤的特異性抗體結合該,利於免疫組織化學(IHC)、Western Blotting等實驗的進行。 (zh)
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- Das HA-Tag ist ein Protein-Tag, der bei der Erzeugung und Charakterisierung von rekombinanten Proteinen verwendet wird. (de)
- HA標籤(HA-tag)是一種基於人流感病毒血凝素(Human influenza hemagglutinin)抗原的蛋白標籤,其化學本質爲一段來自人流感病毒血凝素98-106號氨基酸的短氨基酸序列。使用分子生物學手段將HA標籤序列接合到目的蛋白的一端後,就可以使用抗HA標籤的特異性抗體結合該,利於免疫組織化學(IHC)、Western Blotting等實驗的進行。 (zh)
- Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA-tag, which does not generally appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation and purification of the protein of interest. (en)
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- HA-Tag (de)
- HA-tag (en)
- HA標籤 (zh)
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