Enterovirus cis-acting replication element is a small RNA hairpin in the coding region of protein 2C as the site in PV1(M) RNA that is used as the primary template for the in vitro . The first step in the replication of the plus-stranded poliovirus RNA is the synthesis of a complementary minus strand. This process is initiated by the covalent attachment of uridine monophosphate (UMP) to the terminal protein VPg, yielding and .
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