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Anelosimus buffoni is a species of tangle-web spiders in the Madagascar group of the genus Anelosimus. It is known only from Périnet Special Reserve, Toamasina Province, Madagascar. Adults of the species have a total length of 4.1mm, and is physically similar to several other Madagascar group spiders, particularly and . It is primarily diagnosed using genetics. The species is named for Georges-Louis Leclerc, Comte de Buffon.
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