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The 2000 NCAA Division I softball season, play of college softball in the United States organized by the National Collegiate Athletic Association (NCAA) at the Division I level, began in February 2000. The season progressed through the regular season, many conference tournaments and championship series, and concluded with the 2000 NCAA Division I softball tournament and 2000 Women's College World Series. The Women's College World Series, consisting of the eight remaining teams in the NCAA Tournament and held in Oklahoma City at ASA Hall of Fame Stadium, ended on May 29, 2000.