U.S. Route 301 (US 301) is a spur of U.S. Route 1 running through the South Atlantic States. It runs 1,099 miles (1,769 km) from Biddles Corner, Delaware at Delaware Route 1 to Sarasota, Florida at U.S. Route 41. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Middletown, Delaware; Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, and Sarasota, Florida.