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Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.

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  • Fluoreszenzlebensdauer-Mikroskopie (de)
  • Fluorescence-lifetime imaging microscopy (en)
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  • Die Fluoreszenzlebensdauer-Mikroskopie (englisch fluorescence lifetime imaging microscopy, FLIM) ist ein Fluoreszenz-basiertes bildgebendes Verfahren der Mikroskopie. Im Gegensatz zu anderen fluoreszenzmikroskopischen Verfahren beruht sie nicht auf einer Messung der Fluoreszenzintensität, sondern auf der Messung der unterschiedlichen Lebensdauern der angeregten Zustände fluoreszierender Moleküle. Die Fluoreszenzlebensdauer-Mikroskopie wird insbesondere in Verbindung mit der Konfokalmikroskopie und der Multiphotonenmikroskopie angewendet. (de)
  • Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. (en)
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  • Die Fluoreszenzlebensdauer-Mikroskopie (englisch fluorescence lifetime imaging microscopy, FLIM) ist ein Fluoreszenz-basiertes bildgebendes Verfahren der Mikroskopie. Im Gegensatz zu anderen fluoreszenzmikroskopischen Verfahren beruht sie nicht auf einer Messung der Fluoreszenzintensität, sondern auf der Messung der unterschiedlichen Lebensdauern der angeregten Zustände fluoreszierender Moleküle. Die Fluoreszenzlebensdauer-Mikroskopie wird insbesondere in Verbindung mit der Konfokalmikroskopie und der Multiphotonenmikroskopie angewendet. (de)
  • Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The fluorescence lifetime (FLT) of the fluorophore, rather than its intensity, is used to create the image in FLIM. Fluorescence lifetime depends on the local micro-environment of the fluorophore, thus precluding any erroneous measurements in fluorescence intensity due to change in brightness of the light source, background light intensity or limited photo-bleaching. This technique also has the advantage of minimizing the effect of photon scattering in thick layers of sample. Being dependent on the micro-environment, lifetime measurements have been used as an indicator for pH, viscosity and chemical species concentration. (en)
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