About: End-sequence profiling

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End-sequence profiling (ESP) (sometimes "Paired-end mapping (PEM)") is a method based on sequence-tagged connectors developed to facilitate de novo genome sequencing to identify high-resolution copy number and structural aberrations such as inversions and translocations.

Attributes	Values
rdf:type	software
rdfs:label	End-sequence profiling (en)
rdfs:comment	End-sequence profiling (ESP) (sometimes "Paired-end mapping (PEM)") is a method based on sequence-tagged connectors developed to facilitate de novo genome sequencing to identify high-resolution copy number and structural aberrations such as inversions and translocations. (en)
foaf:depiction
dcterms:subject	DNA sequencing Molecular biology Molecular biology techniques Laboratory techniques
Wikipage page ID	45458060 (xsd:integer)
Wikipage revision ID	1052067107 (xsd:integer)
Link from a Wikipage to another Wikipage	Bacterial artificial chromosomes Insertion (genetics) Polymerase chain reaction Copy-number variation Deletion (genetics) DNA sequencing Molecular biology Molecular biology techniques Chromosomal inversion Chromosomal translocation Chromosome abnormality Bacterial artificial chromosome Laboratory techniques Chromosome abnormalities dbr:Wikt:_de_novo
sameAs	End-sequence profiling End-sequence profiling End-sequence profiling End-sequence profiling
dbp:wikiPageUsesTemplate	dbt:Reflist dbt:Use_dmy_dates
thumbnail	wiki-commons:Special:FilePath/Working_flow_ESP.jpeg?width=300
has abstract	End-sequence profiling (ESP) (sometimes "Paired-end mapping (PEM)") is a method based on sequence-tagged connectors developed to facilitate de novo genome sequencing to identify high-resolution copy number and structural aberrations such as inversions and translocations. Briefly, the target genomic DNA is isolated and partially digested with restriction enzymes into large fragments. Following size-fractionation, the fragments are cloned into plasmids to construct artificial chromosomes such as bacterial artificial chromosomes (BAC) which are then sequenced and compared to the reference genome. The differences, including orientation and length variations between constructed chromosomes and the reference genome, will suggest copy number and structural aberration. (en)
gold:hypernym	Method
prov:wasDerivedFrom	wikipedia-en:End-sequence_profiling?oldid=1052067107&ns=0
page length (characters) of wiki page	13538 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:End-sequence_profiling
is Link from a Wikipage to another Wikipage of	Oncogenomics Deletion (genetics) Halobacterium noricense Bacterial artificial chromosome Cancer genome sequencing
is foaf:primaryTopic of	wikipedia-en:End-sequence_profiling

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