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Denaturation midpoint of a protein is defined as the temperature (Tm) or concentration of denaturant (Cm) at which both the folded and unfolded states are equally populated at equilibrium (assuming two-state protein folding). Tm is often determined using a thermal shift assay.

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  • Denaturation midpoint (en)
  • 变性中点 (zh)
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  • 变性中点（英語：denaturation midpoint）是指在蛋白质变性的二态模型中折叠态向伸展态转变的中点状态，此时折叠态与伸展态的比例相等。这时对应的温度称为中点温度（Tm），对应的浓度则称为中点浓度（Cm）。 理论上，中点时折叠态与伸展态的自由能相等。不过在实际情形中，由于热容的差异导致折叠态的自由能更低。此时，使两者自由能相等的温度被称为特征温度（characteristic temperature，To）。 (zh)
  • Denaturation midpoint of a protein is defined as the temperature (Tm) or concentration of denaturant (Cm) at which both the folded and unfolded states are equally populated at equilibrium (assuming two-state protein folding). Tm is often determined using a thermal shift assay. (en)
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  • Denaturation midpoint of a protein is defined as the temperature (Tm) or concentration of denaturant (Cm) at which both the folded and unfolded states are equally populated at equilibrium (assuming two-state protein folding). Tm is often determined using a thermal shift assay. If the widths of the folded and unfolded wells are assumed to be equal both these states will have identical free energies at the midpoint. However, for natural proteins this is not the case. There is an inherent asymmetry as evidenced by the difference in heat capacities between them - the folded ensemble has a lower heat capacity (in other words, lower fluctuations thus indicating a narrower well) than the unfolded ensemble. This would mean that the free energy of the folded state is lower at the denaturation midpoint than the unfolded state. In such a scenario, the temperature at which both the wells have identical free energies is termed the characteristic temperature (To). (en)
  • 变性中点（英語：denaturation midpoint）是指在蛋白质变性的二态模型中折叠态向伸展态转变的中点状态，此时折叠态与伸展态的比例相等。这时对应的温度称为中点温度（Tm），对应的浓度则称为中点浓度（Cm）。 理论上，中点时折叠态与伸展态的自由能相等。不过在实际情形中，由于热容的差异导致折叠态的自由能更低。此时，使两者自由能相等的温度被称为特征温度（characteristic temperature，To）。 (zh)
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