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About: Bimolecular fluorescence complementation     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:WikicatProtein–proteinInteractionAssays, within Data Space : dbpedia.org associated with source document(s)

Type: companyyago:WikicatBiologicalTechniquesAndToolsyago:Ability105616246yago:AbsorptionIndicator114598383yago:Abstraction100002137yago:Appraisal105733583yago:Assay105738625yago:BasicCognitiveProcess105701944yago:Classification105732756yago:Cognition100023271yago:ColoringMaterial114984973yago:Dye114985383yago:Fluorescein114987695yago:Indicator114917208yago:Know-how105616786yago:Material114580897yago:Matter100020827yago:Method105660268yago:Part113809207yago:PhysicalEntity100001930yago:Process105701363yago:PsychologicalFeature100023100yago:Relation100031921yago:Substance100019613yago:Technique105665146yago:WikicatFluorescentDyesyago:WikicatProteinMethodsyago:WikicatProtein–proteinInteractionAssays New Facet based on Instances of this Class

Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the f