The 2000–01 Macedonian Football Cup was the 9th season of Macedonia's football knockout competition. FK Sloga Jugomagnat were the defending champions, having won their second title the year before after reaching their fifth Final in a row. The 2000–01 champions were FK Pelister who beat the defending champions to claim their first title after twice been runners-up in the first 2 Macedonian Cups in 1992–93 & 1993–94.