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- The Duquenois reagent used in the Rapid Modified Duquenois–Levine test (also known as the simple Rapid Duquenois Test), is an established screening test for the presence of cannabis. The test was initially developed in the 1930s by the French Medical Biochemist, Pierre Duquénois (1904–1986), and was adopted in the 1950s by the United Nations as the preferred test for cannabis, and originally claimed to be specific to cannabis. After several modifications, it became known as the Duquenois–Levine test. However, in the 1960s and 70s various studies showed that the test was not specific to cannabis. In 1973, the Supreme Court of Wisconsin ruled the D–L test insufficient evidence for demonstrating that a substance was cannabis, specifically noting that the D–L tests used "are not exclusive or specific for marijuana." The test is one of several forms of modern cannabis drug testing. (en)
- La prueba rápida modificada de Duquenois-Levine es una prueba química probada que indica la presencia de marihuana. El reactivo de Duquenois-Levine, utilizado en esta prueba, puede ser preparado agregando 10 g de vainillina y 5 mL de acetaldehído a 500 mL de etanol. Esta prueba es efectuada colocando aproximadamente 10 a 20 mg de una sustancia objetivo en un tubo de ensayo de vidrio, luego se agrega 10 gotas del reactivo de Duquenois. Después de agitar, se agrega 10 gotas de ácido clorhídrico concentrado, y el tubo es agitado nuevamente. Se registra cualquier color que resulta después del paso de agregar ácido clorhídrico. A continuación se agregan 20 gotas de cloroformo (o un solvente similar), y el contenido del tubo es mezclado en un mezclador de vórtice, luego se le permite reposar y separar en dos fases. Se registra cualquier color que se transfiera a la fase orgánica. La marihuana se vuelve púrpura con la adición del reactivo de Duquenois-Levine y ácido clorhídrico. Al agregarse el solvente orgánico, el color púrpura se transfiere a la fase orgánica, indicando una prueba positiva para cannabinoides.
* Paso 1 - adición del reactivo de Duquenois a un extracto de éter petróleo seco
* Paso 2 - adición de ácido clorhídrico
* Paso 3 - adición de cloroformo (es)
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- The Duquenois reagent used in the Rapid Modified Duquenois–Levine test (also known as the simple Rapid Duquenois Test), is an established screening test for the presence of cannabis. The test was initially developed in the 1930s by the French Medical Biochemist, Pierre Duquénois (1904–1986), and was adopted in the 1950s by the United Nations as the preferred test for cannabis, and originally claimed to be specific to cannabis. After several modifications, it became known as the Duquenois–Levine test. However, in the 1960s and 70s various studies showed that the test was not specific to cannabis. In 1973, the Supreme Court of Wisconsin ruled the D–L test insufficient evidence for demonstrating that a substance was cannabis, specifically noting that the D–L tests used "are not exclusive or s (en)
- La prueba rápida modificada de Duquenois-Levine es una prueba química probada que indica la presencia de marihuana. El reactivo de Duquenois-Levine, utilizado en esta prueba, puede ser preparado agregando 10 g de vainillina y 5 mL de acetaldehído a 500 mL de etanol. La marihuana se vuelve púrpura con la adición del reactivo de Duquenois-Levine y ácido clorhídrico. Al agregarse el solvente orgánico, el color púrpura se transfiere a la fase orgánica, indicando una prueba positiva para cannabinoides.
* Paso 1 - adición del reactivo de Duquenois a un extracto de éter petróleo seco
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- Prueba rápida modificada de Duquenois-Levine (es)
- Duquenois–Levine reagent (en)
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