The genus Actinomadura is one of four genera of Actinomycetota that belong to the family Thermomonosporaceae. It contains aerobic, Gram-positive, non-acid-fast, non-motile, chemo-organotrophic actinomycetes that produce well-developed, non-fragmenting vegetative mycelia and aerial hyphae that differentiate into surface-ornamented spore chains. These chains are of various lengths and can be straight, hooked or spiral. The genus currently comprises over 70 species with validly published names with standing in nomenclature, although the species status of some strains remains uncertain, and further comparative studies are needed.