The 2001 Pan American Race Walking Cup was held in Cuenca, Azuay, Ecuador on 27–28 October. The track of the Cup runs in the Avenida España, Parque Calderón. The event was held jointly with the South American Race Walking Championships, featuring the men's 35 kilometres race rather than the 50 kilometres. Complete results, medal winners until 2011, and the results for the Mexican athletes were published.