The 1965 Tulane Green Wave football team was an American football team that represented Tulane University during the 1965 NCAA University Division football season as a member of the Southeastern Conference. In their fourth year under head coach Tommy O'Boyle, the team compiled a 2–8 record. This marked the final season Tulane competed as a member of the Southeastern Conference as University President Herbert E. Longenecker announced their formal withdrawal from SEC competition, effective June 1966.