The 1964 Cal Poly Mustangs football team represented California Polytechnic State College—now known as California Polytechnic State University, San Luis Obispo—as a member of the California Collegiate Athletic Association (CCAA) during the 1964 NCAA College Division football season. Led by third-year head coach Sheldon Harden, Cal Poly compiled an overall record of 0–10 with a mark of 0–5 in conference play, placing last out of six teams in the CCAA. The Mustangs played home games at Mustang Stadium in San Luis Obispo, California.