This HTML5 document contains 93 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n22http://dbpedia.org/resource/Wikt:
dbpedia-dehttp://de.dbpedia.org/resource/
dctermshttp://purl.org/dc/terms/
yago-reshttp://yago-knowledge.org/resource/
dbohttp://dbpedia.org/ontology/
n21http://dbpedia.org/resource/File:
foafhttp://xmlns.com/foaf/0.1/
n19https://global.dbpedia.org/id/
yagohttp://dbpedia.org/class/yago/
dbthttp://dbpedia.org/resource/Template:
rdfshttp://www.w3.org/2000/01/rdf-schema#
freebasehttp://rdf.freebase.com/ns/
n16http://commons.wikimedia.org/wiki/Special:FilePath/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
owlhttp://www.w3.org/2002/07/owl#
dbpedia-frhttp://fr.dbpedia.org/resource/
wikipedia-enhttp://en.wikipedia.org/wiki/
dbphttp://dbpedia.org/property/
dbchttp://dbpedia.org/resource/Category:
provhttp://www.w3.org/ns/prov#
xsdhhttp://www.w3.org/2001/XMLSchema#
wikidatahttp://www.wikidata.org/entity/
goldhttp://purl.org/linguistics/gold/
dbrhttp://dbpedia.org/resource/

Statements

Subject Item
dbr:MAP11
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Methods_to_investigate_protein–protein_interactions
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Saccharomyces_cerevisiae
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Protein–protein_interaction_screening
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Protein–protein_interaction
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:SDS-PAGE
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Tandem_affinity_purification
rdf:type
yago:PsychologicalFeature100023100 yago:BasicCognitiveProcess105701944 yago:Classification105732756 yago:Process105701363 yago:Assay105738625 dbo:TopicalConcept yago:Appraisal105733583 yago:WikicatProtein–proteinInteractionAssays yago:Abstraction100002137 yago:Cognition100023271
rdfs:label
Tandem affinity purification Tandem Affinity Purification Méthode TAP
rdfs:comment
Tandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract from a cell only the protein of interest, in complex with any other proteins it interacted with. TAP uses two types of agarose beads that bind to the protein of interest and that can be separated from the cell lysate by centrifugation, without disturbing, denaturing or contaminating the involved complexes. To enable the protein of interest to bind to the beads, it is tagged with a designed piece, the TAP tag. Die Tandem Affinity Purification (TAP) beschreibt die Proteinreinigung unter Verwendung zweier verschiedener chromatographischer Aufreinigungsmethoden. Bei einer Verwendung zweier verschiedener Protein-Tags können beispielsweise zwei verschiedene Affinitätschromatographien durchgeführt werden. Im erweiterten Sinne umfasst die Tandem Affinity Purification alle seriellen Aufreinigungsverfahren, die auf der Affinität des aufzureinigenden Materials zur stationären Phase beruhen. La méthode TAP ((en)TAP-TAG) est une méthode de purification par immunoprécipitation de complexes protéiques basée sur l'utilisation de protéines chimériques portant une étiquette composée de deux domaines d'affinité (historiquement CBP et protéine A). Le principe de la méthode repose sur l'utilisation successive de deux colonnes d'affinité, les complexes étant détachés de la première colonne par un clivage protéolytique, et par compétition de la seconde colonne. Etapes de purification
foaf:depiction
n16:Taptag_simple.png
dcterms:subject
dbc:Protein–protein_interaction_assays dbc:Biochemical_separation_processes
dbo:wikiPageID
9931632
dbo:wikiPageRevisionID
1077305769
dbo:wikiPageWikiLink
dbr:N-terminal dbr:Proteomics dbr:Fusion_protein dbr:Calmodulin dbr:Protein_tag dbr:Epitope dbr:In_vivo dbr:IgG dbr:Transient_protein_interactions dbr:Lysis dbr:Immunoprecipitation dbr:Mass_spectrometry dbc:Protein–protein_interaction_assays dbr:EGTA_(chemical) dbr:Agarose dbr:Tobacco_etch_virus_protease dbc:Biochemical_separation_processes dbr:Protein_complex dbr:Yeast_two-hybrid n21:Taptag_simple.png dbr:Yeast dbr:Protein–protein_interaction dbr:Immunoglobulin_G dbr:TEV_protease n22:specificity dbr:European_Molecular_Biology_Laboratory dbr:Photo-reactive_amino_acid_analog dbr:Plasmids dbr:Bond_cleavage dbr:Protein_A dbr:Affinity_(pharmacology) dbr:Translate dbr:SDS-PAGE dbr:C-terminus
owl:sameAs
wikidata:Q893051 dbpedia-de:Tandem_Affinity_Purification yago-res:Tandem_affinity_purification n19:52pM1 dbpedia-fr:Méthode_TAP freebase:m.02pxcdv
dbp:wikiPageUsesTemplate
dbt:Citation_needed dbt:Reflist
dbo:thumbnail
n16:Taptag_simple.png?width=300
dbo:abstract
La méthode TAP ((en)TAP-TAG) est une méthode de purification par immunoprécipitation de complexes protéiques basée sur l'utilisation de protéines chimériques portant une étiquette composée de deux domaines d'affinité (historiquement CBP et protéine A). Le principe de la méthode repose sur l'utilisation successive de deux colonnes d'affinité, les complexes étant détachés de la première colonne par un clivage protéolytique, et par compétition de la seconde colonne. Etapes de purification La première étape consiste à incuber l'échantillon avec des billes d'immunoglobulines de type G (IgG) qui vont lier la protéine A. Une protase TEV est utilisée ensuite pour détacher la protéine d'intérêt. La deuxième étape consiste à incuber le complexe protéique obtenu à l'étape précédente avec des billes de calmoduline (CM) et de calcium (Ca2+). La calmoduline va permettre de lier spécifiquement la protéine d'intérêt. L'EGTA est ensuite utilisé pour purifier la solution et obtenir uniquement la protéine d'intérêt, car l'EGTA va se lier aux ions calcium. Die Tandem Affinity Purification (TAP) beschreibt die Proteinreinigung unter Verwendung zweier verschiedener chromatographischer Aufreinigungsmethoden. Bei einer Verwendung zweier verschiedener Protein-Tags können beispielsweise zwei verschiedene Affinitätschromatographien durchgeführt werden. Im erweiterten Sinne umfasst die Tandem Affinity Purification alle seriellen Aufreinigungsverfahren, die auf der Affinität des aufzureinigenden Materials zur stationären Phase beruhen. Tandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract from a cell only the protein of interest, in complex with any other proteins it interacted with. TAP uses two types of agarose beads that bind to the protein of interest and that can be separated from the cell lysate by centrifugation, without disturbing, denaturing or contaminating the involved complexes. To enable the protein of interest to bind to the beads, it is tagged with a designed piece, the TAP tag. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of three components: a calmodulin binding peptide (CBP), TEV protease cleavage site, and two Protein A domains, which bind tightly to IgG (making a TAP tag a type of epitope tag). Many other tag/bead/eluent combinations have been proposed since the TAP principle was first published.
gold:hypernym
dbr:Technique
prov:wasDerivedFrom
wikipedia-en:Tandem_affinity_purification?oldid=1077305769&ns=0
dbo:wikiPageLength
10398
foaf:isPrimaryTopicOf
wikipedia-en:Tandem_affinity_purification
Subject Item
dbr:Tap
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
dbo:wikiPageDisambiguates
dbr:Tandem_affinity_purification
Subject Item
dbr:EGTA_(chemical)
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Erin_K._O'Shea
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:C2orf16
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Human_interactome
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:FAM227B
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Polyhistidine-tag
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:STK24
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
Subject Item
dbr:Tandem_Affinity_Purification
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
dbo:wikiPageRedirects
dbr:Tandem_affinity_purification
Subject Item
dbr:Tap_tag
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
dbo:wikiPageRedirects
dbr:Tandem_affinity_purification
Subject Item
dbr:TAP-tag
dbo:wikiPageWikiLink
dbr:Tandem_affinity_purification
dbo:wikiPageRedirects
dbr:Tandem_affinity_purification
Subject Item
wikipedia-en:Tandem_affinity_purification
foaf:primaryTopic
dbr:Tandem_affinity_purification