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Statements

Subject Item
dbr:Long-range_restriction_mapping
rdfs:label
Long-range restriction mapping
rdfs:comment
Long-range restriction mapping is an alternative genomic mapping technique to short-range, also called fine-scale mapping. Both forms utilize restriction enzymes in order to decipher the previously unknown order of DNA segments; the main difference between the two being the amount of DNA that comprises the final map. The unknown DNA is broken into many smaller fragments by these restriction enzymes at specific sites on the molecule, and then the fragments can later be analyzed by their individual sizes. A final long-range map can span hundreds to thousands of kilobytes of genetic data at many different loci.
dct:subject
dbc:Restriction_enzymes dbc:Genomics_techniques
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67315994
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1120703473
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Long-range restriction mapping is an alternative genomic mapping technique to short-range, also called fine-scale mapping. Both forms utilize restriction enzymes in order to decipher the previously unknown order of DNA segments; the main difference between the two being the amount of DNA that comprises the final map. The unknown DNA is broken into many smaller fragments by these restriction enzymes at specific sites on the molecule, and then the fragments can later be analyzed by their individual sizes. A final long-range map can span hundreds to thousands of kilobytes of genetic data at many different loci. The long-range maps cover very large genomics regions in order to display the physical relationship of DNA segments targeted by restriction enzymes. These restriction sites are an integral component to the formation of long-range mapping. Genetic linkage data can be combined with gel electrophoresis procedures to provide gene order as well as distance on chromosomes. To accomplish this, the genetic linkage information is used to create a theory-based hypothesis: one that can be tested with gel electrophoresis and extended DNA sequencing protocols.
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wikipedia-en:Long-range_restriction_mapping?oldid=1120703473&ns=0
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wikipedia-en:Long-range_restriction_mapping
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