About: Radioimmunoprecipitation assay buffer

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Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. The stronger detergents in RIPA buffer (such as SDS) cause greater protein denaturation and decrease protein-protein interactions.

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  • Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. The stronger detergents in RIPA buffer (such as SDS) cause greater protein denaturation and decrease protein-protein interactions. (en)
  • RIPA裂解液（英語：Radioimmunoprecipitation assay buffer，全称放射免疫沉淀法缓冲液）是一种用于裂解细胞或组织的，常用于放射性免疫沉淀分析（RIPA）。此缓冲液的变性性能比或曲拉通X-100裂解缓冲液更强，因为它包含离子型去垢剂SDS和脱氧胆酸钠作为破坏核膜，提取核内物质的活性成分。RIPA缓冲液在实验时能提供低的背景，但也会使激酶变性。 (zh)
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  • Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. The stronger detergents in RIPA buffer (such as SDS) cause greater protein denaturation and decrease protein-protein interactions. (en)
  • RIPA裂解液（英語：Radioimmunoprecipitation assay buffer，全称放射免疫沉淀法缓冲液）是一种用于裂解细胞或组织的，常用于放射性免疫沉淀分析（RIPA）。此缓冲液的变性性能比或曲拉通X-100裂解缓冲液更强，因为它包含离子型去垢剂SDS和脱氧胆酸钠作为破坏核膜，提取核内物质的活性成分。RIPA缓冲液在实验时能提供低的背景，但也会使激酶变性。 (zh)
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  • Radioimmunoprecipitation assay buffer (en)
  • RIPA裂解液 (zh)
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