dbo:abstract
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- o-Nitrophenyl-β-D-galactopyranosid (ONPG) ist ein artifizielles Glycosid und chromogenes Substrat für β-Galactosidasen, z. B. das vom lacZ Gen von Escherichia coli codierte Enzym. (de)
- ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it is unable to act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed. (en)
- L'orthonitrophényl-β-galactoside (ou 2-nitrophényl-β-D-galactopyrannoside, ONPG) est un hétéroside de type bêta-galactoside, dont l'aglycone est l' (ONP) et la partie glucidique est constituée par un galactose. L'ONPG est incolore. Son hydrolyse par une β-galactosidase libère du galactose et de l' (ONP), composé de couleur jaune en milieu alcalin. (fr)
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dbo:alternativeName
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- 2-Nitrophenylgalactoside, Ortho-Nitrophenyl-β-D-Galactopyranoside (en)
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dbo:iupacName
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- (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol (en)
- 2-Nitrophenyl β-(D)-galactopyranoside (en)
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- 2331 (xsd:nonNegativeInteger)
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- ortho-Nitrophenyl-β-galactoside (en)
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rdfs:comment
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- o-Nitrophenyl-β-D-galactopyranosid (ONPG) ist ein artifizielles Glycosid und chromogenes Substrat für β-Galactosidasen, z. B. das vom lacZ Gen von Escherichia coli codierte Enzym. (de)
- L'orthonitrophényl-β-galactoside (ou 2-nitrophényl-β-D-galactopyrannoside, ONPG) est un hétéroside de type bêta-galactoside, dont l'aglycone est l' (ONP) et la partie glucidique est constituée par un galactose. L'ONPG est incolore. Son hydrolyse par une β-galactosidase libère du galactose et de l' (ONP), composé de couleur jaune en milieu alcalin. (fr)
- ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate. (en)
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rdfs:label
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- O-Nitrophenyl-β-D-galactopyranosid (de)
- Ortho-nitrophényl-β-galactoside (fr)
- Ortho-Nitrophenyl-β-galactoside (en)
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- ortho-Nitrophenyl-β-galactoside (en)
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