| dbo:abstract
|
- o-Nitrophenyl-β-D-galactopyranosid (ONPG) ist ein artifizielles Glycosid und chromogenes Substrat für β-Galactosidasen, z. B. das vom lacZ Gen von Escherichia coli codierte Enzym. (de)
- ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it is unable to act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed. (en)
- L'orthonitrophényl-β-galactoside (ou 2-nitrophényl-β-D-galactopyrannoside, ONPG) est un hétéroside de type bêta-galactoside, dont l'aglycone est l' (ONP) et la partie glucidique est constituée par un galactose. L'ONPG est incolore. Son hydrolyse par une β-galactosidase libère du galactose et de l' (ONP), composé de couleur jaune en milieu alcalin. (fr)
|
| dbo:alternativeName
|
- 2-Nitrophenylgalactoside, Ortho-Nitrophenyl-β-D-Galactopyranoside (en)
|
| dbo:iupacName
|
- (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol (en)
- 2-Nitrophenyl β-(D)-galactopyranoside (en)
|
| dbo:thumbnail
| |
| dbo:wikiPageID
| |
| dbo:wikiPageLength
|
- 2331 (xsd:nonNegativeInteger)
|
| dbo:wikiPageRevisionID
| |
| dbo:wikiPageWikiLink
| |
| dbp:imagefile
| |
| dbp:iupacname
| |
| dbp:name
|
- ortho-Nitrophenyl-β-galactoside (en)
|
| dbp:othernames
| |
| dbp:pin
| |
| dbp:wikiPageUsesTemplate
| |
| dcterms:subject
| |
| gold:hypernym
| |
| rdf:type
| |
| rdfs:comment
|
- o-Nitrophenyl-β-D-galactopyranosid (ONPG) ist ein artifizielles Glycosid und chromogenes Substrat für β-Galactosidasen, z. B. das vom lacZ Gen von Escherichia coli codierte Enzym. (de)
- L'orthonitrophényl-β-galactoside (ou 2-nitrophényl-β-D-galactopyrannoside, ONPG) est un hétéroside de type bêta-galactoside, dont l'aglycone est l' (ONP) et la partie glucidique est constituée par un galactose. L'ONPG est incolore. Son hydrolyse par une β-galactosidase libère du galactose et de l' (ONP), composé de couleur jaune en milieu alcalin. (fr)
- ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate. (en)
|
| rdfs:label
|
- O-Nitrophenyl-β-D-galactopyranosid (de)
- Ortho-nitrophényl-β-galactoside (fr)
- Ortho-Nitrophenyl-β-galactoside (en)
|
| owl:sameAs
| |
| prov:wasDerivedFrom
| |
| foaf:depiction
| |
| foaf:isPrimaryTopicOf
| |
| foaf:name
|
- ortho-Nitrophenyl-β-galactoside (en)
|
| is dbo:wikiPageRedirects
of | |
| is dbo:wikiPageWikiLink
of | |
| is foaf:primaryTopic
of | |
