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Optical mapping is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence. Originally developed by Dr. David C. Schwartz and his lab at NYU in the 1990s this method has since been integral to the assembly process of many large-scale sequencing projects for both microbial and eukaryotic genomes. Later technologies use DNA melting, DNA competitive binding or enzymatic labelling in order to create the optical mappings.

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  • Optical mapping is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence. Originally developed by Dr. David C. Schwartz and his lab at NYU in the 1990s this method has since been integral to the assembly process of many large-scale sequencing projects for both microbial and eukaryotic genomes. Later technologies use DNA melting, DNA competitive binding or enzymatic labelling in order to create the optical mappings. (en)
  • La cartographie optique est une technique pour l'élaboration d'une carte de sites de restriction à partir de fragments d'ADN. Les cartes produites permettent de visualiser la composition génomique ou chromosomique à une échelle plus générale que le séquençage, et offrent un profil (sous forme de zébrures) de cette suite de données génétiques. La technique a été mise au point dans les années 1990 par Dr. David Schwartz et son équipe à la Haute École de New York. Depuis, la méthode a joué un rôle fondamental aux étapes d'assemblage de nombreux grands projets de séquençage de génomes microbiens et eucaryotiques. (fr)
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  • Optical mapping is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence. Originally developed by Dr. David C. Schwartz and his lab at NYU in the 1990s this method has since been integral to the assembly process of many large-scale sequencing projects for both microbial and eukaryotic genomes. Later technologies use DNA melting, DNA competitive binding or enzymatic labelling in order to create the optical mappings. (en)
  • La cartographie optique est une technique pour l'élaboration d'une carte de sites de restriction à partir de fragments d'ADN. Les cartes produites permettent de visualiser la composition génomique ou chromosomique à une échelle plus générale que le séquençage, et offrent un profil (sous forme de zébrures) de cette suite de données génétiques. La technique a été mise au point dans les années 1990 par Dr. David Schwartz et son équipe à la Haute École de New York. Depuis, la méthode a joué un rôle fondamental aux étapes d'assemblage de nombreux grands projets de séquençage de génomes microbiens et eucaryotiques. (fr)
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  • Cartographie optique (fr)
  • Optical mapping (en)
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