James Johnstone (4 July 1801 – 24 February 1888) was a Scottish Liberal Party politician. He was the oldest of 8 sons of James Raymond Johnstone (died 1830) of Alva, who was the son of John Johnstone (1734–1795). He was elected at a as the Member of Parliament (MP) for Clackmannanshire and Kinross-shire.He was returned unopposed in 1852, and did not contest the 1857 election.