Graphis tetralocularis is a species of crustose lichen in the family Graphidaceae. Found in Rwanda, it was formally described as a new species in 2005 by Christina Bock and Markus Hauck. The type specimen was collected by the first author from Akagera National Park at an altitude of 1,369 m (4,491 ft); here it was found growing on a twig of a Nuxia floribunda tree. It is only known to occur at the type locality, which is a dry forest dominated by the trees Nuxia floribunda, , and Strychnos usambarensis. The lichen has a thin, smooth, whitish grey to grey-green crustose thallus. The specific epithet tetralocularis refers to the unusual morphology of its four-chambered ascospores. Graphis tetralocularis contains trace amounts of atranorin, a secondary chemical that can be detected using the
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