In enzymology, a formate—dihydrofolate ligase (EC 6.3.4.17) is an enzyme that catalyzes the chemical reaction ATP + formate + dihydrofolate ADP + phosphate + 10-formyldihydrofolate The 3 substrates of this enzyme are ATP, formate, and dihydrofolate, whereas its 3 products are ADP, phosphate, and . This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The systematic name of this enzyme class is formate:dihydrofolate ligase (ADP-forming).
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