Fluorescence guided surgery (FGS), also called fluorescence image-guided surgery, or in the specific case of tumor resection, fluorescence guided resection, is a medical imaging technique used to detect fluorescently labelled structures during surgery. Similarly to standard image-guided surgery, FGS has the purpose of guiding the surgical procedure and providing the surgeon of real time visualization of the operating field. When compared to other medical imaging modalities, FGS is cheaper and superior in terms of resolution and number of molecules detectable. As a drawback, penetration depth is usually very poor (100 μm) in the visible wavelengths, but it can reach up to 1–2 cm when excitation wavelengths in the near infrared are used.
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