Gloucester and Macquarie was an electoral district of the Legislative Assembly in the Australian state of New South Wales in the first and second Parliaments (1856-1859), named after Gloucester and Macquarie counties on the Mid North Coast. It was abolished in 1859 with Macquarie, the north-east of Gloucester and the Macleay River area forming the new district of Electoral district of Hastings (New South Wales), while the rest of Gloucester was split between Lower Hunter, Northumberland and The Williams.
