The Cayuga and Susquehanna Railroad was a railroad in the state of New York, in the United States. Its line ran from Ithaca, New York to Owego, New York. It was founded in 1829 and began operations in 1834. The Delaware, Lackawanna and Western Railroad (Lackawanna) leased the company in 1855, but it remained in existence as a non-operating subsidiary. It was conveyed to Conrail in the bankruptcy of the Erie Lackawanna Railway, successor to the Lackawanna, in 1976.