The 1965 Colorado Buffaloes football team was an American football team that represented the University of Colorado as a member of the Big Eight Conference during the 1965 NCAA University Division football season. Led by third-year head coach Eddie Crowder, the Buffaloes compiled an overall record of 6–2–2 with a mark of 4–2–1 in conference play, placing third in the Big 8. Colorado played their home games on campus at Folsom Field in Boulder, Colorado.