The 1959 Denver Pioneers football team was an American football team that represented the University of Denver as a member of the Skyline Conference during the 1959 NCAA University Division football season. In its fifth season under head coach John Roning, the team compiled an overall record of 2–8 record with a mark of 2–5 against conference opponents, tied for fifth place in the Skyline, and was outscored by a total of 230 to 104.