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About: Propidium iodide     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:WikicatQuaternaryAmmoniumCompounds, within Data Space : dbpedia.org associated with source document(s)

Type: Thingdul:ChemicalObjectchemical substanceChemical compoundyago:WikicatAromaticCompoundsyago:WikicatDNA-bindingSubstancesyago:WikicatNucleotidesyago:WikicatStainingDyesyago:AbsorptionIndicator114598383yago:Abstraction100002137yago:AromaticCompound114610088yago:Chemical114806838yago:ColoringMaterial114984973yago:Compound114818238yago:Dye114985383yago:Ester114850483yago:Fluorescein114987695yago:Halide114904359yago:Hydrocarbon114911057yago:Indicator114917208yago:Iodide114922371yago:Material114580897yago:Matter100020827yago:Nucleotide114964590yago:OrganicCompound114727670yago:Part113809207yago:PhysicalEntity100001930yago:QuaternaryAmmoniumCompound114691231yago:Relation100031921yago:WikicatIodideschemical compoundyago:Salt115010703yago:Substance100019613yago:WikicatFluorescentDyesyago:WikicatPhenanthridineDyesyago:WikicatQuaternaryAmmoniumCompounds New Facet based on Instances of this Class

Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). After binding DNA, the quantum yield of PI is enhanced 20-30 fold, and the excitation/emission maximum of PI is shifted to 535 nm (green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic