The 2000–01 Divizia B was the 61st season of the second tier of the Romanian football league system. The format has been maintained to two series, each of them having 18 teams. At the end of the season, the winners of the series promoted to Divizia A and the last six places from both series relegated to Divizia C. Two more teams relegated this season due to the reduction of team in the next season, from 36 (2x18) to 32 (2x16). A promotion play-off was played between the 13th and 14th-placed in the Divizia A and the runners-up of the Divizia B series.