"7942"^^ . . "Geobacillus stearothermophilus"@en . . "Geobacillus stearothermophilus (bis 2001 als Bacillus stearothermophilus eingeordnet) ist ein sporenbildendes, grampositives st\u00E4bchenf\u00F6rmiges Bakterium. Die Zellen besitzen durchschnittlich 0,6 \u2013 1 \u00B5m Durchmesser und eine L\u00E4nge zwischen 2 und 3,5 \u00B5m. Die optimale Wachstumstemperatur liegt zwischen 50 und 65 \u00B0C, damit z\u00E4hlt Geobacillus stearothermophilus zu den thermophilen Organismen. Bei Temperaturen unter 30 \u00B0C wachsen die Zellen nicht mehr. Geobacillus stearothermophilus dient zur Erzeugung des Enzyms BstBI."@de . "8156765"^^ . . . . . . "\u0627\u0644\u0639\u0635\u0648\u064A\u0629 \u0627\u0644\u062F\u0647\u0646\u064A\u0629 \u0623\u0644\u064A\u0641\u0629 \u0627\u0644\u062D\u0631\u0627\u0631\u0629 (\u0628\u0627\u0644\u0644\u0627\u062A\u064A\u0646\u064A\u0629: Bacillus stearothermophilus \u0623\u0648 Geobacillus stearothermophilus) \u0630\u0627\u062A \u0634\u0643\u0644 \u0639\u0635\u0648\u064A\u060C \u0628\u0643\u062A\u064A\u0631\u064A\u0627 \u0625\u064A\u062C\u0627\u0628\u064A\u0629 \u0627\u0644\u063A\u0631\u0627\u0645 \u0648\u0639\u0636\u0648 \u0641\u064A \u0645\u062A\u064A\u0646\u0627\u062A \u0627\u0644\u062C\u062F\u0627\u0631."@ar . . . . . "DNA polymerase I"@en . . "Bacillus stearothermophilus"@es . . "Thermostable Group II Intron Reverse Transcriptase GsI-IIC"@en . . . . "Geobacillus stearothermophilus"@de . . "Geobacillus stearothermophilus"@ca . "lightgrey"@en . "Geobacillus stearothermophilus (Bacillus stearothermophilus) es una bacteria Gram-positiva con forma de bacilo que se encuadra en el filo Firmicutes. Es una bacteria term\u00F3fila extensamente distribuida en el suelo, manantiales calientes y sedimentos oce\u00E1nicos y es causa de descomposici\u00F3n de los productos alimenticios. Es usada com\u00FAnmente como organismo de validaci\u00F3n en los estudios de esterilizaci\u00F3n. Lo anterior debido a que su degradaci\u00F3n como organismo es cuando se alcanza una temperatura de 121 \u00B0C, presenta una alta resistencia a temperaturas menores. Lo que la hace un buen indicador. En los autoclaves de vapor se utiliza una ampolla con esta bacteria para hacer una prueba de validaci\u00F3n biol\u00F3gica del proceso de esterilizaci\u00F3n respecto a las condiciones te\u00F3ricas de 15 minutos a 121 \u00B0C con 1 atm de presi\u00F3n. La o las ampollas se distribuyen en el autoclave (se trata de demostrar una esterilizaci\u00F3n uniforme de todo el equipo). Se le da el ciclo a validar de esterilizado efectivo (por ejemplo: 15, 17, 20, etc. minutos). El ciclo puede o no incluir carga si queremos validar el ciclo y distribuci\u00F3n del equipo y si queremos validar la carga de trabajo. Concluido el ciclo, la o las ampollas son incubadas a 60 \u00B0C por 48 horas para ver si se desarrolla la bacteria de control. Normalmente las ampollas tienen un indicador de fenolftale\u00EDna o alg\u00FAn tipo de indicador que var\u00EDa de color por desarrollo de la bacteria que modifica el pH de la ampolla. El viraje habitual es de violeta a amarillo, si luego de la incubaci\u00F3n permanece violeta el ciclo de esterilizado ha sido efectivo. Se debe incubar un control positivo sin esterilizar para verificar el correcto el resultado por cambio de color. En caso de viraje de la o las ampollas se deber\u00E1 aumentar el tiempo de esterilizado, revisi\u00F3n de fugas, presi\u00F3n obtenida, cantidad de carga, etc. para volver a realizar la validaci\u00F3n del proceso. Para la validez estad\u00EDstica del proceso se deber\u00E1 considerar al menos un estudio en triplicado bajo las mismas condiciones de operaci\u00F3n. \n* Datos: Q136876"@es . . . . "Geobacillus"@en . . . "Bacillus stearothermophilus"@it . "lightgrey"@en . . . . "Geobacillus stearothermophilus (abans anomenat Bacillus stearothermophilus) \u00E9s un bacteri amb forma de bacil, gram positiu, membre del f\u00EDlum dels Firmicuts. \u00C9s un microorganisme term\u00F2fil i es troba \u00E0mpliament distribu\u00EFt al s\u00F2l, aig\u00FCes termals, sediments oce\u00E0nics i \u00E9s el causant de que molts productes alimentaris es facin malb\u00E9. Creixer\u00E0 dins d'un rang de temperatura de 30 a 75 \u00B0C. Algunes soques s\u00F3n capaces d'oxidar el mon\u00F2xid de carboni en condicions aer\u00F2biques."@ca . . "Geobacillus stearothermophilus"@en . . . . "Geobacillus stearothermophilus (previously Bacillus stearothermophilus) is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota. The bacterium is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products. It will grow within a temperature range of 30 to 75 \u00B0C. Some strains are capable of oxidizing carbon monoxide aerobically. It is commonly used as a challenge organism for sterilization validation studies and periodic check of sterilization cycles. The biological indicator contains spores of the organism on filter paper inside a vial. After sterilizing, the cap is closed, an ampule of growth medium inside of the vial is crushed and the whole vial is incubated. A color and/or turbidity change indicates the results of the sterilization process; no change indicates that the sterilization conditions were achieved, otherwise the growth of the spores indicates that the sterilization process has not been met. Recently a fluorescent-tagged strain, Rapid Readout(tm), is being used for verifying sterilization, since the visible blue fluorescence appears in about one-tenth the time needed for pH-indicator color change, and an inexpensive light sensor can detect the growing colonies. Biological indicators are used in conjunction with chemical indicators and to validate sterilization processes. It was first described in 1920 as Bacillus stearothermophilus, but, together with Bacillus thermoglucosidasius, it was reclassified as a member of the genus Geobacillus in 2001."@en . "TRT"@en . "\u0627\u0644\u0639\u0635\u0648\u064A\u0629 \u0627\u0644\u062F\u0647\u0646\u064A\u0629 \u0623\u0644\u064A\u0641\u0629 \u0627\u0644\u062D\u0631\u0627\u0631\u0629 (\u0628\u0627\u0644\u0644\u0627\u062A\u064A\u0646\u064A\u0629: Bacillus stearothermophilus \u0623\u0648 Geobacillus stearothermophilus) \u0630\u0627\u062A \u0634\u0643\u0644 \u0639\u0635\u0648\u064A\u060C \u0628\u0643\u062A\u064A\u0631\u064A\u0627 \u0625\u064A\u062C\u0627\u0628\u064A\u0629 \u0627\u0644\u063A\u0631\u0627\u0645 \u0648\u0639\u0636\u0648 \u0641\u064A \u0645\u062A\u064A\u0646\u0627\u062A \u0627\u0644\u062C\u062F\u0627\u0631."@ar . . . "Geobacillus stearothermophilus"@uk . "E1C9K5"@en . . "Geobacillus stearothermophilus (Bacillus stearothermophilus) es una bacteria Gram-positiva con forma de bacilo que se encuadra en el filo Firmicutes. Es una bacteria term\u00F3fila extensamente distribuida en el suelo, manantiales calientes y sedimentos oce\u00E1nicos y es causa de descomposici\u00F3n de los productos alimenticios. En caso de viraje de la o las ampollas se deber\u00E1 aumentar el tiempo de esterilizado, revisi\u00F3n de fugas, presi\u00F3n obtenida, cantidad de carga, etc. para volver a realizar la validaci\u00F3n del proceso. \n* Datos: Q136876"@es . . . "Bacillus stearothermophilus\u306F\u30012021\u5E74\u73FE\u5728\u306FGeobacillus stearothermophilus\u3068\u547C\u3070\u308C\u308B\u300155-60\u2103\u3067\u3088\u304F\u751F\u80B2\u3059\u308B\u901A\u6027\u5ACC\u6C17\u6027\u7D30\u83CC\u3067\u3042\u308B\u3002 \u98DF\u54C1\u52A0\u5DE5\u6642\u306B\u6025\u901F\u306A\u5897\u6B96\u3092\u793A\u3059\u3002"@ja . . . . "PolA"@en . . "Bacillus stearothermophilus (o Geobacillus stearothermophilus) \u00E8 un batterio a bastoncello, Gram-positivo, membro del phylum Firmicutes. Questo batterio \u00E8 termofilo, in quanto ha la capacit\u00E0 di riprodursi a temperature di circa 57 \u00B0C. Esso \u00E8 ampiamente distribuito nei suoli, nelle sorgenti di acqua calda, nei sedimenti oceanici ed \u00E8 una causa della decomposizione dei prodotti alimentari."@it . . . . . "Bacillus stearothermophilus"@ja . . . . . "Geobacillus stearothermophilus est une bact\u00E9rie \u00E0 Gram positif en forme de b\u00E2tonnet. Elle est mobile, produit des spores et est thermophile. On l'utilise g\u00E9n\u00E9ralement pour v\u00E9rifier le bon fonctionnement de st\u00E9rilisateurs \u00E0 la vapeur comme l'autoclave, puisque les bact\u00E9ries sporulantes sont plus r\u00E9sistantes en g\u00E9n\u00E9ral et que les thermophiles tol\u00E8rent bien la chaleur. Ainsi, s'il reste des cellules de Geobacillus stearothermophilus et/ou des spores de cet organisme qui sont encore viables apr\u00E8s avoir chauff\u00E9 dans l'autoclave \u00E0 121 \u00B0C pendant 15 minutes, on sait que l'autoclave ne fonctionne pas bien."@fr . . . . . . "Geobacillus stearothermophilus \u2014 \u043F\u0430\u043B\u0438\u0447\u043A\u043E\u0432\u0438\u0434\u043D\u0430 \u0433\u0440\u0430\u043C-\u043F\u043E\u0437\u0438\u0442\u0438\u0432\u043D\u0430 \u0431\u0430\u043A\u0442\u0435\u0440\u0456\u044F, \u0447\u043B\u0435\u043D \u0442\u0438\u043F\u0443 Firmicutes. \u0426\u0456 \u0431\u0430\u043A\u0442\u0435\u0440\u0456\u0457 \u2014 \u0442\u0435\u0440\u043C\u043E\u0444\u0456\u043B\u0438, \u0449\u043E \u0448\u0438\u0440\u043E\u043A\u043E \u0440\u043E\u0437\u043F\u043E\u0432\u0441\u044E\u0434\u0436\u0435\u043D\u0456 \u0432 \u0491\u0440\u0443\u043D\u0442\u0456, \u0433\u0430\u0440\u044F\u0447\u0438\u0445 \u0434\u0436\u0435\u0440\u0435\u043B\u0430\u0445 \u0442\u0430 \u043E\u043A\u0435\u0430\u043D\u0441\u044C\u043A\u0438\u0445 \u043E\u043F\u0430\u0434\u0430\u0445. \u0412\u043E\u043D\u0438 \u0436 \u2014 \u043E\u0434\u043D\u0430 \u0437 \u043F\u0440\u0438\u0447\u0438\u043D \u043F\u0441\u0443\u0432\u0430\u043D\u043D\u044F \u0445\u0430\u0440\u0447\u043E\u0432\u043E\u0457 \u043F\u0440\u043E\u0434\u0443\u043A\u0446\u0456\u0457."@uk . . "Geobacillus stearothermophilus"@en . . "Bacillus stearothermophilus\u306F\u30012021\u5E74\u73FE\u5728\u306FGeobacillus stearothermophilus\u3068\u547C\u3070\u308C\u308B\u300155-60\u2103\u3067\u3088\u304F\u751F\u80B2\u3059\u308B\u901A\u6027\u5ACC\u6C17\u6027\u7D30\u83CC\u3067\u3042\u308B\u3002 \u98DF\u54C1\u52A0\u5DE5\u6642\u306B\u6025\u901F\u306A\u5897\u6B96\u3092\u793A\u3059\u3002"@ja . . "E2GM63"@en . "\u0639\u0635\u0648\u064A\u0629 \u062F\u0647\u0646\u064A\u0629 \u0623\u0644\u064A\u0641\u0629 \u0627\u0644\u062D\u0631\u0627\u0631\u0629"@ar . . . . . "2"^^ . "Bacillus stearothermophilus (o Geobacillus stearothermophilus) \u00E8 un batterio a bastoncello, Gram-positivo, membro del phylum Firmicutes. Questo batterio \u00E8 termofilo, in quanto ha la capacit\u00E0 di riprodursi a temperature di circa 57 \u00B0C. Esso \u00E8 ampiamente distribuito nei suoli, nelle sorgenti di acqua calda, nei sedimenti oceanici ed \u00E8 una causa della decomposizione dei prodotti alimentari. \u00C8 comunemente utilizzato come organismo campione per gli studi di validazione della sterilizzazione e per le verifiche periodiche dei cicli di sterilizzazione. L'indicatore biologico \u00E8 costituito da spore dell'organismo su carta da filtro in una fiala. Dopo la sterilizzazione il campione \u00E8 sigillato, rompendo un'ampolla di mezzo di coltura posta all'interno, e la fiala viene incubata. Un cambiamento di colore o di torbidit\u00E0 indica il risultato del processo di sterilizzazione: se non ci sono cambiamenti, sono state raggiunte condizioni di sterilit\u00E0, altrimenti la crescita delle spore indica che il processo di sterilizzazione non \u00E8 avvenuto correttamente."@it . . . "Geobacillus stearothermophilus"@en . . "6"^^ . . . "Geobacillus stearothermophilus est une bact\u00E9rie \u00E0 Gram positif en forme de b\u00E2tonnet. Elle est mobile, produit des spores et est thermophile. On l'utilise g\u00E9n\u00E9ralement pour v\u00E9rifier le bon fonctionnement de st\u00E9rilisateurs \u00E0 la vapeur comme l'autoclave, puisque les bact\u00E9ries sporulantes sont plus r\u00E9sistantes en g\u00E9n\u00E9ral et que les thermophiles tol\u00E8rent bien la chaleur. Ainsi, s'il reste des cellules de Geobacillus stearothermophilus et/ou des spores de cet organisme qui sont encore viables apr\u00E8s avoir chauff\u00E9 dans l'autoclave \u00E0 121 \u00B0C pendant 15 minutes, on sait que l'autoclave ne fonctionne pas bien."@fr . . . . . "Geobacillus stearothermophilus"@fr . "Geobacillus stearothermophilus \u2014 \u043F\u0430\u043B\u0438\u0447\u043A\u043E\u0432\u0438\u0434\u043D\u0430 \u0433\u0440\u0430\u043C-\u043F\u043E\u0437\u0438\u0442\u0438\u0432\u043D\u0430 \u0431\u0430\u043A\u0442\u0435\u0440\u0456\u044F, \u0447\u043B\u0435\u043D \u0442\u0438\u043F\u0443 Firmicutes. \u0426\u0456 \u0431\u0430\u043A\u0442\u0435\u0440\u0456\u0457 \u2014 \u0442\u0435\u0440\u043C\u043E\u0444\u0456\u043B\u0438, \u0449\u043E \u0448\u0438\u0440\u043E\u043A\u043E \u0440\u043E\u0437\u043F\u043E\u0432\u0441\u044E\u0434\u0436\u0435\u043D\u0456 \u0432 \u0491\u0440\u0443\u043D\u0442\u0456, \u0433\u0430\u0440\u044F\u0447\u0438\u0445 \u0434\u0436\u0435\u0440\u0435\u043B\u0430\u0445 \u0442\u0430 \u043E\u043A\u0435\u0430\u043D\u0441\u044C\u043A\u0438\u0445 \u043E\u043F\u0430\u0434\u0430\u0445. \u0412\u043E\u043D\u0438 \u0436 \u2014 \u043E\u0434\u043D\u0430 \u0437 \u043F\u0440\u0438\u0447\u0438\u043D \u043F\u0441\u0443\u0432\u0430\u043D\u043D\u044F \u0445\u0430\u0440\u0447\u043E\u0432\u043E\u0457 \u043F\u0440\u043E\u0434\u0443\u043A\u0446\u0456\u0457."@uk . . "G. stearothermophilus"@en . "1082242966"^^ . "Geobacillus stearothermophilus (previously Bacillus stearothermophilus) is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota. The bacterium is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products. It will grow within a temperature range of 30 to 75 \u00B0C. Some strains are capable of oxidizing carbon monoxide aerobically. It is commonly used as a challenge organism for sterilization validation studies and periodic check of sterilization cycles. The biological indicator contains spores of the organism on filter paper inside a vial. After sterilizing, the cap is closed, an ampule of growth medium inside of the vial is crushed and the whole vial is incubated. A color and/or turbidity change indicates the"@en . "Geobacillus stearothermophilus (bis 2001 als Bacillus stearothermophilus eingeordnet) ist ein sporenbildendes, grampositives st\u00E4bchenf\u00F6rmiges Bakterium. Die Zellen besitzen durchschnittlich 0,6 \u2013 1 \u00B5m Durchmesser und eine L\u00E4nge zwischen 2 und 3,5 \u00B5m. Die optimale Wachstumstemperatur liegt zwischen 50 und 65 \u00B0C, damit z\u00E4hlt Geobacillus stearothermophilus zu den thermophilen Organismen. Bei Temperaturen unter 30 \u00B0C wachsen die Zellen nicht mehr. Die Bildung der Endosporen erfolgt terminal, dabei wird die Bakterienzelle keulenf\u00F6rmig aufgetrieben. Die widerstandsf\u00E4higen Sporen werden in B\u00F6den, in hei\u00DFen Quellen, im W\u00FCstensand, in arktischen Gew\u00E4ssern, im Meeresboden, in Lebensmitteln und in Kompost vorgefunden, keimen aber nur unter g\u00FCnstigen Bedingungen aus. Das Bakterium ist f\u00FCr Menschen und Tiere nicht pathogen, also nicht krankheitserregend. Geobacillus stearothermophilus dient als Pr\u00FCfkeim f\u00FCr den Erfolg von thermischen Sterilisationsprozessen (zum Beispiel Autoklavieren). Hierf\u00FCr werden zum Beispiel Glasampullen verwendet, die ein fl\u00FCssiges N\u00E4hrmedium, Sporen von Geobacillus stearothermophilus und einen Farbindikator enthalten. Die Ampullen werden wie \u00FCblich autoklaviert und anschlie\u00DFend zusammen mit einer nicht autoklavierten Kontroll-Ampulle bei etwa 60 \u00B0C f\u00FCr mehrere Tage inkubiert. Stoffwechselaktivit\u00E4t der Zellen wird durch einen Farbumschlag des Indikators angezeigt. Erfolgreich sterilisierte Ampullen ver\u00E4ndern ihre F\u00E4rbung daher nicht. Eine andere Anwendungsform sind Sporenstreifen, dabei handelt es sich um Filterpapierstreifen, auf denen die Endosporen aufgebracht wurden. Zur Kontrolle der Dampfsterilisation gibt man die Sporenstreifen in den Autoklaven, anschlie\u00DFend werden sie in eine N\u00E4hrl\u00F6sung gelegt und wie oben angegeben inkubiert. Wenn die Sterilisation erfolgreich verlaufen ist, erfolgt kein Wachstum. Geobacillus stearothermophilus dient zur Erzeugung des Enzyms BstBI."@de . . "Geobacillus stearothermophilus"@en . . "Geobacillus stearothermophilus (abans anomenat Bacillus stearothermophilus) \u00E9s un bacteri amb forma de bacil, gram positiu, membre del f\u00EDlum dels Firmicuts. \u00C9s un microorganisme term\u00F2fil i es troba \u00E0mpliament distribu\u00EFt al s\u00F2l, aig\u00FCes termals, sediments oce\u00E0nics i \u00E9s el causant de que molts productes alimentaris es facin malb\u00E9. Creixer\u00E0 dins d'un rang de temperatura de 30 a 75 \u00B0C. Algunes soques s\u00F3n capaces d'oxidar el mon\u00F2xid de carboni en condicions aer\u00F2biques. Va ser descrit per primera vegada al 1920 com a Bacillus stearothermophilus, per\u00F2 al 2001, juntament amb el Bacillus thermoglucosidasius van ser classificats de nou com membres del g\u00E8nere Geobacillus."@ca .