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Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. This bond can form before or after embedding the cells into slides. A reaction occurs between the antigen and antibody, causing this label to become visible under the microscope. Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell.

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  • Imunoelektronová mikroskopie je metoda používaná v elektronové mikroskopii pro určení virové infekce. Metoda využívá schopnost protilátek vázat se na specifické receptory (antigeny) na povrchu buňky. Protilátka způsobuje shlukování virových částic, které jsou jinak rozptýleny. Protilátky jsou menší než viry a mají jiný tvar, takže lze viry identifikovat. Poprvé byla imunoelektronová mikroskopie použita v roce 1941 při zkoumání viru tabákové mozaiky. V šedesátých letech tuto metodu využívala viroložka June Almeida při výzkumu virů, který vedl k objevu koronavirů. (cs)
  • Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. This bond can form before or after embedding the cells into slides. A reaction occurs between the antigen and antibody, causing this label to become visible under the microscope. Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. (en)
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  • Imunoelektronová mikroskopie je metoda používaná v elektronové mikroskopii pro určení virové infekce. Metoda využívá schopnost protilátek vázat se na specifické receptory (antigeny) na povrchu buňky. Protilátka způsobuje shlukování virových částic, které jsou jinak rozptýleny. Protilátky jsou menší než viry a mají jiný tvar, takže lze viry identifikovat. Poprvé byla imunoelektronová mikroskopie použita v roce 1941 při zkoumání viru tabákové mozaiky. V šedesátých letech tuto metodu využívala viroložka June Almeida při výzkumu virů, který vedl k objevu koronavirů. (cs)
  • Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. This bond can form before or after embedding the cells into slides. A reaction occurs between the antigen and antibody, causing this label to become visible under the microscope. Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. (en)
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  • Imunoelektronová mikroskopie (cs)
  • Immune electron microscopy (en)
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