Photoactivation is a technique used in biological research to specifically activate cellular players (proteins, nucleic acids, small molecules) by a flash of light in order to study processes in cells. The basic principle is to bring a photoactivatable agent (e.g. a small molecule modified with a light-responsive group, proteins tagged with an ) to cells, tissue or even living animals and specifically control the activity by illumination

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  • Photoactivation is a technique used in biological research to specifically activate cellular players (proteins, nucleic acids, small molecules) by a flash of light in order to study processes in cells. The basic principle is to bring a photoactivatable agent (e.g. a small molecule modified with a light-responsive group, proteins tagged with an ) to cells, tissue or even living animals and specifically control the activity by illumination Light is a well-suited external trigger for these types of experiments since it is non-invasive and does not influence normal cellular processes (care has to be taken when using light in the ultra-violet part of the spectrum to avoid DNA damage. Furthermore, light offers high spatial and temporal control. Usually, the activation stimulus comes from a laser or a UV lamp and can be incorporated into the same microscope used for monitoring of the effect. All these advantages have led to the development of a wide variety of different photoactivatable probes. Even though the light-induced activation step is usually irreversible, reversible changes can be induced in a number of photoswitches, which will not be discussed in detail here. (en)
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  • Photoactivation is a technique used in biological research to specifically activate cellular players (proteins, nucleic acids, small molecules) by a flash of light in order to study processes in cells. The basic principle is to bring a photoactivatable agent (e.g. a small molecule modified with a light-responsive group, proteins tagged with an ) to cells, tissue or even living animals and specifically control the activity by illumination (en)
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  • Photoactivatable probes (en)
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