| dbo:abstract
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- Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self-splicing) can occur under high-salt conditions in vitro. However, assistance from proteins is required for in vivo splicing. In contrast to group I introns, intron excision occurs in the absence of GTP and involves the formation of a lariat, with an A-residue branchpoint strongly resembling that found in lariats formed during splicing of nuclear pre-mRNA. It is hypothesized that pre-mRNA splicing (see spliceosome) may have evolved from group II introns, due to the similar catalytic mechanism as well as the structural similarity of the Group II Domain V substructure to the U6/U2 extended snRNA. Finally, their ability to site-specifically insert into DNA sites has been exploited as a tool for biotechnology. For example, group II introns can be modified to make site-specific genome insertions and deliver cargo DNA such as reporter genes or lox sites (en)
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- 14774 (xsd:nonNegativeInteger)
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- full secondary structure of group II intron (en)
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| dbp:extra
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| dbp:interpro
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- IPR013597 (en)
- IPR024937 (en)
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| dbp:name
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- Domain X (en)
- Group II catalytic intron, D1-D4 (en)
- Group II catalytic intron, D5 (en)
- Group II intron, maturase-specific (en)
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| dbp:pdb
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- 4 (xsd:integer)
- 6 (xsd:integer)
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| dbp:pfam
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- PF01348 (en)
- PF08388 (en)
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| dbp:rfam
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| dbp:rfamClan
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- Domain_X (en)
- GIIM (en)
- Intron_gpII (en)
- group-II-D1D4 (en)
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| rdfs:comment
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- Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self-splicing) can occur under high-salt conditions in vitro. However, assistance from proteins is required for in vivo splicing. In contrast to group I introns, intron excision occurs in the absence of GTP and involves the formation of a lariat, with an A-residue branchpoint strongly resembling that found in lariats formed during splicing of nuclear pre-mRNA. It is hypothesized that pre-mRNA splicing (see spliceosome) may have evolved from group II introns, due to the similar catalytic mechanism as well as the structural similarity of the Group II Domain V substructure to the U6/U2 extended snRNA. Finally, their ability to (en)
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