Sainte-Marie is a city in the province of Quebec, Canada. It is the seat of the Municipalité régionale de la Nouvelle-Beauce, in Chaudière-Appalaches. The population was 13,134 as of the Canada 2021 Census, and was 95.7% French-speaking as of 2021. It is located 59 kilometres (37 mi) south-east of Quebec City, on the Chaudière River.