Barra del Colorado Wildlife Refuge is a wildlife refuge, part of the Tortuguero Conservation Area, in Limón Province in the northeastern part of Costa Rica. It is the second largest rain forest preserve in the country and protects areas that contain hot , mangroves, canals and marine areas, bounded in part by the San Juan and Colorado rivers, and to the south the Tortuguero National Park. The village of Tortuguero from which the neighbouring Tortuguero National Park got its name is located on the southern border the refuge, and contains two research stations.