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Agnes Hedwig of Anhalt (12 March 1573, Dessau – 3 November 1616, Sønderborg) was a Princess of Anhalt by birth, an Abbess of Gernrode, and by marriage Electress of Saxony and later Duchess of Schleswig-Holstein-Sonderburg. She was the daughter of Prince Joachim Ernest of Anhalt and his second wife . From 1581 to 1586 she was abbess of the Imperial Abbey of St. Cyriac in Gernrode. Two years later, on 14 February 1588, she married Duke John II of Schleswig-Holstein-Sonderburg; she was his second wife, too. She died in 1616, six years before her husband.