The 1965 Saint John's Johnnies football team was an American football team that represented Saint John's University as a member of the Minnesota Intercollegiate Athletic Conference (MIAC) during the 1965 NAIA football season. In their 13th season under head coach John Gagliardi, the Johnnies compiled an 11–0 record (7–0 against conference opponents) and won the MIAC championship. The team advanced to the NAIA playoff and won the NAIA national championship with a 33–0 victory over Linfield Wildcats in the Champion Bowl. It was the second of four national championships for the Saint John's Johnnies football program under head coach John Gagliardi.