The Tarpon Springs Depot is a former railroad depot built by the Atlantic Coast Line Railroad in 1909, located in downtown Tarpon Springs, Florida at 160 East Tarpon Avenue within the Tarpon Springs Historic District. The former rail line that serviced the depot, originally built by the Orange Belt Railway in 1888 and last used for a series of special excursion trips to nearby Dunedin and back on March 8, 1987, was converted into a section of the Pinellas Trail in the 1990s.