Protein chemical synthesis by native peptide ligation of unprotected peptide segments is an interesting complement and potential alternative to the use of living systems for producing proteins.The synthesis of proteins requires efficient native peptide ligation methods, which enable the chemoselective formation of a native peptide bond in aqueous solution between unprotected peptide segments.The most frequently used technique for synthesizing proteins is Native chemical ligation (NCL). However, alternatives are emerging,one of which is SEA Native Peptide Ligation.
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