The Pucker Up Stakes is an American Thoroughbred horse race for 3-year-old fillies held annually in early September at Arlington Park race track near Chicago, Illinois. A Grade III event, it is contested over a distance of 9 furlongs (1 1/8 miles) on turf. In 2022, it will be moved to Churchill Downs. The race was named in honor of Ada L. Rice's filly, Pucker Up. Pucker Up was one of five champions trained by U.S. Racing Hall of Fame trainer James P. Conway.